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The methods and materials section is where you capture (in paragraph form) your basic procedure and your experimental design strategy. The materials that you used should be obvious as you detail your procedure and experimental design. Do NOT just list your materials, or give a numbered (step 1, step 2, etc.) bulleted procedure. These paragraphs should convey the following information (the items listed are in order of the corresponding rubric):
Your design strategy should clearly address your stated goal. This means that it should be clear from reading your experimental procedure whether or not you should be able to achieve your goal.
It needs to be clearly stated within the section how many replicates (trials) and runs were carried out; it may be necessary to justify these numbers if they are unconventional (for example, if you had 10 replicates because your AAD was high).
Did you do adequate experimentation based on the precision of your data?
It should be clear to the reader that your preliminary testing justifies your final testing strategy; there should not be a major scientific leap going into your final testing (new variables, etc.)
While the procedural steps should not be numbered, the methods and materials section should be written sequentially. The paragraphs should also be clear and accurate, with respect to actual steps you did in the laboratory. While your methods section needs to be thorough, you should also be concise.
Your IV and DV should both be clearly and accurately identified. It should be clear within this section how you manipulated your IV (what were you changing?) and how you measured or quantified your DV.
It is also critical that the experimental set-up is clear from this section. If text does not do an adequate job (subjective to the reader), then a visual aid can be quite useful. If a visual aid is used, then it: must be clear, captioned (as a figure) appropriately, and cited in the text.
All of your experimentally-controlled variables should be clearly identified and quantified (if appropriate) within your methods and materials section. For each control, it should be clear WHY you controlled them and HOW you controlled them.
Your procedure should be detailed enough and have the appropriate clarity that it would be repeatable (thorough and unambiguous) for a reader
If your data collection methods involves statistical analysis, you should mention it here. This analysis should be well justified based on your data and your experiment.